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. 2021 Nov 17;7(47):eabj0829. doi: 10.1126/sciadv.abj0829

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Copyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).

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Fig. 6. — (A) Shannon diversity index of the microbial communities of control free-swimming lungfish skin (C), terrestrialized lungfish skin (TS), terrestrialized cocoon (TC), DNase I–treated terrestrialized lungfish skin (DTS), and DNase I–treated terrestrialized lungfish cocoon (DTC) (n = 3). (B) Microbial community composition at the phylum level of the microbial communities of control free-swimming lungfish skin (C), terrestrialized lungfish skin, terrestrialized cocoon, DNase I–treated terrestrialized lungfish skin, and DNase I–treated terrestrialized lungfish cocoon. (C) Microbial community composition at the family level of the microbial communities of control free-swimming lungfish skin, terrestrialized lungfish skin, terrestrialized cocoon, DNase I–treated terrestrialized lungfish skin, and DNase I–treated terrestrialized lungfish cocoon. (D) Heatmap of the top 36 bacterial genera with differential abundances in the bacterial communities of control free-swimming lungfish skin, terrestrialized lungfish skin, terrestrialized cocoon, DNase I–treated terrestrialized lungfish skin, and DNase I–treated terrestrialized lungfish cocoon (P < 0.05). The heatmap was generated using the online free tool Heatmapper using average linkage as a clustering method followed by the Spearman rank correlation for distance measurement. (E) Mean relative abundance at the genus level of Variovorax sp., Stenotrophomonas sp., Limnobacter sp.; Aeromonas sp., and Methylophilus sp. in the microbial communities of control free-swimming lungfish skin, terrestrialized lungfish skin, terrestrialized cocoon, DNase I–treated terrestrialized lungfish skin, and DNase I–treated terrestrialized lungfish cocoon. Data were analyzed in Quantitative Insights Into Microbial Ecology (QIIME) 1.8. Differential abundances were determined by unpaired Student’s t test. *P < 0.05.