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. 2021 Nov 15;220(12):e202104114. doi: 10.1083/jcb.202104114

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© 2021 Tsuchiya and Goshima

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Figure 8. — Aurora kinases contribute to γ-tubulin-independent MTOC formation in mitosis. (A) Flowchart of MT depolymerization-regrowth assay in prometaphase combined with AID and drug treatment. (B–E) MT regrowth after drug washout in the indicated cell lines and treatment. The cells with undetectable levels of γ-tubulin are marked in red circles. Blue arrows in the right panel indicate the centriole, which retains MTs even after cold/drug treatment, whereas green arrows indicate noncentriolar MTOCs. Scale bars, 10 µm. (F) Timing of ncMTOC formation after nocodazole washout in the indicated cell treatment. n values are listed within each graph.