FIG. 4.

Synthetic phenotypes of the GPA1-A345T and ste2Δ mutations. (A) Growth was assessed at 30 and 38°C for the GPA1⁺ ste2Δ and GPA1-A345T ste2Δ cells that carried the vector control or carried GPA1 or a STE2 allele on a plasmid. Tenfold serial dilutions of each culture were spotted onto selective medium, and duplicate plates were incubated at 30°C for 36 h or at 38°C for 72 h. (B) Microscopic images were obtained for the same strains grown at 30 and 37°C. The cells were cultured in selective liquid medium for 16 h at 30°C and then for an additional 18 h at either 30 or 37°C. GPA1 and GPA1-A345T shown at the top of each panel indicate the chromosomal allele (strains DJ925-1-3 and DJ926-10-3, respectively). Vector, GPA1, STE2, and ste2-T326 indicate the gene present on the plasmid (pJK67, YCpC3, pDB02, and pJBK023, respectively).