Figure 2. Synapse counts are elevated at postnatal day 7 (P7) and reduced at P28 in Tmc1^Δ/Δ;Tmc2^Δ/Δ mice relative to wild-type (WT) mice.

(A–B) Representative image of P7 WT and Tmc1^Δ/Δ;Tmc2^Δ/Δ inner hair cells (IHCs) from 16 kHz region immunostained for anti-Myosin7a (gray), anti-CtBP2 (red), and anti-GluA2 (green). Higher magnification images are shown below. (C–D) P28 WT and Tmc1^Δ/Δ;Tmc2^Δ/Δ IHCs from 16 kHz region. Scale bars: 10 µm (upper) and 5 µm (lower).