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. 2021 Nov 4;10:e69433. doi: 10.7554/eLife.69433

Key resources table.

Reagent type (species) or resource Designation Source or reference Identifiers Additional information
Genetic reagent (Mus musculus) C57B/L6-Cdh23753A>G Derived from Lentz et al., 2010 C57BL6 Lentz et al. Dev. Neurobiol (2010)
Genetic reagent (Mus musculus) 019146 - B6.129-Tmc1tm1.1Ajg/J Available from Jackson Lab, obtained initially from Dr A Griffith (NIH/NIDCD) Tmc1 Targeted (Reporter, Null/Knockout) Kurima et al. Nat Genet. (2002)
Genetic reagent (Mus musculus) 019147 - B6.129-Tmc2tm1.1Ajg/J Available from Jackson Lab, obtained initially from Dr A Griffith (NIH/NIDCD) Tmc2 Targeted (Reporter, Null/Knockout) Kawashima et al. J Clin Invest (2011)
Genetic reagent (Mus musculus) 003929 - BXA4/Pgn-Tmiesr-J/J Available from Jax C57BL/6-Tmiesr (‘spinner’) mice Spontaneous mutation in Tmie Stock No. 000543Mitchem et al. Hum Mol Genet. (2002)
Genetic reagent (Mus musculus) Tmc1Bth Tmc1Bth/Bth mice were obtained from M Hrabé de Angelis and H Fuchs, Institute of Experimental Genetics, Neuherberg, Germany Point mutation at residue 412 (M412K) Vreugde et al. Nat Genet. (2002)
Antibody Anti-CtBP2 (Mouse IgG1 monoclonal) BD Transduction Laboratories Cat #: 612044 Primary antibody, IF (1:200)
Antibody Anti-GluA2 (Mouse IgG2a monoclonal) Millipore Sigma Cat #: MABN1189 Primary antibody, IF (1:2000)
Antibody Anti-MyosinVIIA (Rabbit polyclonal) Proteus Biosciences Cat #: 25–6790 Primary antibody, IF (1:200)
Antibody Anti-Rabbit Alexa Fluor 647 (Donkey polyclonal) Thermo Fisher Scientific Cat #: A-31573 Secondary antibody, IF (1:200)
Antibody Anti-Mouse IgG2a Alexa Fluor 488 (Goat polyclonal) Thermo Fisher Scientific Cat #: A-21131 Secondary antibody, IF (1:1000)
Antibody Anti-Mouse IgG1 Alexa Fluor 546 (Goat polyclonal) Thermo Fisher Scientific Cat #: A-21123 Secondary antibody, IF (1:1000)
Other Vectashield Antifade Vector Laboratories Cat #: H-1000–10 Mounting medium
Other AAV9-PHP.B- CMV-Tmc1e × 1 Wu et al., 2021 Nucleic acid, Titer: 3.9 E + 13 gc/mL
Software, algorithm Imaris https://imaris.oxinst.com/
Software, algorithm ImageJ software http://imagej.nih.gov/ij/
Software, algorithm Eaton-Peabody Laboratories Cochlear Function Test Suite https://www.masseyeandear.org/research/otolaryngology/eaton-peabody-laboratories/engineering-core