Key resources table.
| Reagent type (species) or resource | Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (Mus musculus) | L3-Cas9+ | Kiritsy and Ankley et al. (co-submitted) |
Primary BMDMs immortalized with J2 virus were transduced with Cas9 and single cell cloned | |
| Cell line (M. musculus) | EGFP-Cas9 iBMDMs | This paper | Primary BMDMs from Jackson Stock 026179 were immortalized with J2 virus | |
| Cell line (M. musculus) | sgNdufa1 EGFP-Cas9 iBMDMs | This paper | Cas9+ iBMDMs were transduced with Ndufa1 sgRNA | |
| Cell line (M. musculus) | sgNdufa2 EGFP-Cas9 iBMDMs | This paper | Cas9+ iBMDMs were transduced with Ndufa2 sgRNA | |
| Cell line (M. musculus) | Cas9+ C57BL/6J estradiol-inducible HoxB8 progenitors | This paper | Myeloid progenitors from Jackson stock 026179 were immortalized with HoxB8 retrovirus and maintained with 10 µM estradiol | |
| Cell line (M. musculus) | sgNdufa1 C57BL/6J estradiol-inducible HoxB8 progenitors | This study | Cas9+ HoxB8 cells were transduced with Ndufa1 sgRNA | |
| Cell line (M. musculus) | Cas9+ C57BL/6J estradiol-inducible HoxB8 progenitors | This paper | Myeloid progenitors from Jackson stock 003288 were immortalized with HoxB8 retrovirus and maintained with 10 µM estradiol | |
| Strain, strain background (M. musculus) | C57BL6J | Jackson Laboratories |
Stock 000664 | |
| Strain, strain background (M. musculus) | C7 TCR-transgenic mice (specific for ESAT-6 antigen) |
PMID:18779346 | Mice were donated to and maintained by the Behar laboratory | |
| Peptide, recombinant protein | ESAT-6 peptide (MTEQQW NFAGIEAAA) |
New England Peptide |
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| Recombinant DNA reagent | sgOpti | Addgene | RRID:Addgene_ 85681 | |
| Recombinant DNA reagent | sgOpti with blasticidin and zeocyin selection | Kiritsy and Ankley et al. (co- submitted) |
sgOpti (RRID 85681) was modified with bacterial selection replaced with zeocyin and mammalian selection replaced with blasticidin | |
| Recombinant DNA reagent | sgOpti with hygromycin and kanamycin selection | Kiritsy and Ankley et al. (co-submitted) |
sgOpti (RRID 85681) was modified with bacterial selection replaced with kanamycin and mammalian selection replaced with hygromycin | |
| Recombinant DNA reagent | VSVG | Addgene | RRID:Addgene_8454 | |
| Recombinant DNA reagent | psPax2 | Addgene | RRID:Addgene_12260 | |
| Antibody | MHCII-PE, clone M5/114.15.2 (rat monoclonal) |
BioLegend | RRID:AB_313323 | FC (1:800) |
| Recombinant DNA reagent | Mouse CRISPR KO pooled library (BRIE) | Addgene | RRID:Addgene_7363 | |
| Chemical compound, drug | Rotenone | Sigma | Cat# R8875 | |
| Chemical compound, drug | Oligomycin | Cayman | Cat# 11342 | |
| Chemical compound, drug | CCCP | Cayman | Cat# 25458 | |
| Chemical compound, drug | 1400W | Cayman | Cat# 81520 | |
| Chemical compound, drug | N-Acetyl cysteine | Cayman | Cat# 20261 | |
| Chemical compound, drug | Dimethyloxallyl glycine (DMOG) | Cayman | Cat# 71210 | |
| Chemical compound, drug | UK5099 | Cayman | Cat# 16,980 | |
| Chemical compound, drug | 2-Deoxyglucose (2-DG) | Cayman | Cat# 14325 | |
| Chemical compound, drug | MitoTEMPO hydrate | Cayman | Cat# 16621 | |
| Chemical compound, drug | Sodium azide | Sigma | Cat# S2002 | |
| Chemical compound, drug | Antimycin A | Sigma | Cat# A8674 | |
| Chemical compound, drug |
Pam3CSK4 | Invivogen | Cat# tlrl-pms | |
| Chemical compound, drug |
Linezolid (LZD) | Gift from Clifton Barry (used here PMID:32477361) |
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| Antibody | Purified anti-STAT1 antibody clone A15158C (mouse monoclonal) | BioLegend | Cat# 603701; RRID:AB_2749867 | WB (1:1000) |
| Antibody | Phospho-Stat1 Tyr701, clone 58D6 (rabbit monoclonal) | Cell Signaling Technology |
Cat# 5375; RRID:AB_10860071 | WB (1:1000) |
| Antibody | Purified anti-STAT1 Phospho Ser727 antibody, clone A15158B (mouse monoclonal) | BioLegend | Cat# 686408; RRID:AB_2650782 | WB (1:1000) |
| Antibody | Jak2 XP, clone D2E12 (rabbit monoclonal) | Cell Signaling Technology |
Cat# 4040; RRID:AB_10691469 | WB (1:500) |
| Antibody | Phospho-Jak2 (Tyr1007/1008) antibody (unknown) | Cell Signaling Technology |
Cat# 3771; RRID:AB_330403 | WB (1:500) |
| Antibody | Biotin anti-mouse CD119, IFNγ Rα chain antibody clone 2E2 (Armenian hamster monoclonal) | BioLegend | Cat# 112803; RRID:AB_2123476 | WB (1:1000) |
| Antibody | Anti-mouse β-actin antibody, clone C4 (mouse monoclonal) | Santa Cruz Biotechnology |
Cat# sc-51850; RRID:AB_629337 | WB (1:2000) |
| Antibody | CD274-Bv421 clone 10F.9G2 (rat monoclonal) | BioLegend | RRID:AB_10897097 | FC (1:400) |
| Commercial assay or kit | Zombie Aqua Fixable Viability Kit | BioLegend | Cat# 423101 | FC (1:100) |
| Commercial assay or kit | DNeasy Blood and Tissue Kit | Qiagen | Cat# 69504 | |
| Antibody | CD40 APC anti-mouse CD40 antibody, clone 3/23 (rat monoclonal) | BioLegend | Cat# 124611;RRID:AB_1134081 | FC (1:200) |
| Antibody | Human anti-CD54, clone HCD54 (mouse monoclonal) | BioLegend | Cat# 322718; RRID:AB_2248731 | FC (1:400) |
| Antibody | Human anti-CD40 clone 5C3 (mouse monoclonal) | BioLegend | Cat# 334307; RRID:AB_1186060 | FC (1:400) |
| Antibody | Human anti-CD274, B7-H1, PD-L1, clone 29E (mouse monoclonal) | BioLegend | Cat# 329713; RRID:AB_10901164 | FC (1:400) |
| Antibody | Human anti-HLA-DR antibody, clone L243 (mouse monoclonal) | BioLegend | Cat# 307657; RRID:AB_2572100 | FC (1:400) |
| Antibody | Anti-mouse IFNγ antibody (rat monoclonal) | BioLegend | Cat# 505807; RRID:AB_315401 | FC (1:200) |
| Peptide, recombinant protein | Murine IL-12 | PeproTech | Cat# 210-12 | |
| Peptide, recombinant protein | Human GM-CSF | PeproTech | Cat# 300-03 | |
| Peptide, recombinant protein | Human IFN gamma | PeproTech | Cat# 300-02 | |
| Peptide, recombinant protein | Murine TNF | PeproTech | Cat# 315-01A | |
| Antibody | Anti-IL4 clone: 11B11 (rat monoclonal) | BioLegend | RRID:AB_2750407 | Neutralization (1:500) |
| Antibody | Goat anti-rabbit HRP (goat polyclonal) | Invitrogen | Cat# 31460 | WB (1:1000) |
| Antibody | Goat anti-mouse HRP (goat polyclonal) | Invitrogen | Cat# 31430 | WB (1:1000) |
| Commercial assay or kit | One-Step RT PCR Kit | Qiagen | Cat# 210215 | |
| Commercial assay or kit | Luna Universal One-Step RT-qPCR Kit | NEB | Cat# E3005 | |
| Commercial assay or kit | Trizol | Thermo Fisher Scientific |
Cat# 15596026 | |
| Commercial assay or kit | CellTiter-Glo 2.0 Cell Viability Assay | Promega | Cat# G9241 | |
| Commercial assay or kit | Seahorse assay media | Agilent | Cat# 103575-100 | |
| Software, algorithm | MAGECK | PMID:25476604 |
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| Peptide, recombinant protein | Interferon gamma | BioLegend | Cat# 575308 | |
| Commercial assay or kit | MojoSORT – Mouse CD4 Naïve T cell Isolation Kit | BioLegend | Cat# 480040 | |
| Commercial assay or kit | MojoSort Human CD14 Nanobeads | BioLegend | Cat# BioLegend 480093 | |
| Commercial assay or kit | IL6 ELISA-max | BioLegend | Cat# 431301 | |
| Commercial assay or kit | TNF ELISA-max | BioLegend | Cat# 430901 | |
| Commercial assay or kit | Human IL1b | R&D Systems | Cat# DY201 | |
| Commercial assay or kit | Human TNF-alpha DuoSet ELISA | R&D Systems | Cat# DY210 | |
| Commercial assay or kit | Greiss reagent | Promega | G2930 | |
| Sequence-based reagent | All oligonucleotide sequences are contained in Supplementary file 1. | This paper | All oligonucleotide sequences are contained in Supplementary file 1 |