Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Mus musculus) | C57Bl/6J | The Jackson Laboratory | MGI:3028467 RRID:MGI:3028467 |
|
| Strain, strain background (Mus musculus) | B6.129P2-Lyz2tm1(cre)Ifo/JC57Bl/6J | Clausen et al., 1999 | MGI:1934631 RRID:IMSR_JAX:004781 |
|
| Strain, strain background (Mus musculus) | B6.129S4(Cg)-Arntltm1Weit/J, C57Bl/6J | Storch et al., 2007 | MGI: J:124157 | |
| Strain, strain background (Mus musculus) | B6.129S4-Clocktm1Rep/J, C57Bl/6J | Debruyne et al., 2006 | MGI: J:109634 | |
| Strain, strain background (Mus musculus) | B6.129S6-Per2tm1Jt/J, C57Bl/6J | Yoo et al., 2004 | MGI: J:89256 | |
| biological sample (Escherichia coli O55:B5) | LPS | Sigma-Aldrich | L2880 | LOTs 102M4017V, 032M4082V, 063M4041V |
| Antibody | CD11b Microbeads (rat monoclonal) |
Miltenyi | Cat# 130-049-601 | MACS (1:5) |
| Antibody | α-mCD3 APC-Cy7 (rat monoclonal) |
Biolegend | Cat# 100221 RRID:AB_2057374 |
FACS (1:200) |
| Antibody | α-mCD4 FITC (rat monoclonal) |
Biolegend | Cat# 100405 RRID:AB_312690 |
FACS (1:500) |
| Antibody | α-mCD8 PE-Cy7 (rat monoclonal) |
Biolegend | Cat# 100721 RRID:AB_312760 |
FACS (1:200) |
| Antibody | α-mCD11b PE-Cy7 (rat monoclonal) |
Ebioscience | Cat# 25-0112 RRID:AB_469587 |
FACS (1:200) |
| Antibody | α-mCD11c APC (armenian hamster monoclonal) |
Biolegend | Cat# 117309 RRID:AB_313778 |
FACS (1:200) |
| Antibody | α-mCD14 PE (rat monoclonal) |
Biolegend | Cat# 123309 RRID:AB_940582 |
FACS (1:500) |
| Antibody | α-mCD19 Pacific Blue (rat monoclonal) |
Biolegend | Cat# 115526 RRID:AB_493341 |
FACS (1:200) |
| Antibody | α-mCD45 PerCP-Cy5.5 (rat monoclonal) |
Biolegend | Cat# 103131 RRID:AB_893344 |
FACS (1:200) |
| Antibody | α-mLy6C Brilliant Violet 421 (rat monoclonal) |
Biolegend | Cat# 128033 RRID:AB_2562351 |
FACS (1:200) |
| Antibody | α-mLy6G FITC (rat monoclonal) |
BD | Cat# 561105 RRID:AB_394207 |
FACS (1:1000) |
| Antibody | α-mF4/80 APC (rat monoclonal) |
Biolegend | Cat# 123115 RRID:AB_893493 |
FACS (1:100) |
| Antibody | α-mNK1.1 PE (mouse monoclonal) |
Biolegend | Cat# 108707 RRID:AB_313394 |
FACS (1:500) |
| Antibody | VD eFluor 506 | eBioscience | Cat# 65-0866-14 |
FACS (1:500) |
| Antibody | VD eFluor 780 | eBioscience | Cat# 65-0865-14 |
FACS (1:1000) |
| Antibody | Rb α-mBmal1 (rabbit polyclonal) |
kind gift from Michael Brunner | WB (1:500) | |
| Antibody | Rb α-mClock (rabbit polyclonal) |
Bethyl Laboratories | Cat# A302-618A RRID:AB_10555233 |
WB (1:1000) |
| Antibody | Mm α-mβ-Actin (mouse monoclonal) |
Sigma-Aldrich | Cat# A-5441 RRID:AB_476744 |
WB (1:100000) |
| Antibody | Gt α-mIgG-HRP (goat polyclonal) |
SantaCruz Biotechnology | Cat# sc-2005 RRID:AB_631736 |
WB (1:1000) |
| Antibody | DK α-rbIgG-HRP (donkey polyclonal) |
SantaCruz Biotechnology | Cat# sc-2305 RRID:AB_641180 |
WB (1:1000) |
| Commercial assay or kit | Mouse IL-6 ELISA Ready-SET-Go! | Ebioscience | Cat# 88-7064-88 RRID:AB_2574990 |
|
| Commercial assay or kit | ProcartaPlex, Mix & Match, mouse 13-plex | Ebioscience | EPX 130-24021-801 | |
| Sequence-based reagent | mCry1 | Qiagen | QT00117012 | |
| Sequence-based reagent | mCry2 | Qiagen | QT00168868 | |
| Sequence-based reagent | mDpb | Qiagen | QT00103089 | |
| Sequence-based reagent | mNr1d1 | Qiagen | QT00164556 | |
| Sequence-based reagent | mNpas2 | Qiagen | QT00108647 |
|
| Sequence-based reagent | mPer1 | Qiagen | QT00113337 | |
| Sequence-based reagent | mPer2 | Qiagen | QT00198366 | |
| Sequence-based reagent | mGapdh fw | This paper | qPCR primer | ACGGGAAGCTCACTGGCATGGCCTT |
| Sequence-based reagent | mGapdh rv | This paper | qPCR primer | CATGAGGTCCACCACCCTGTTGCTG |
| Sequence-based reagent | mBmal1 fw | This paper | qPCR primer | GGACACAGACAAAGATGACCC |
| Sequence-based reagent | mBmal1 rv | This paper | qPCR primer | TTTTGTCCCGACGCCTCTTT |
| Sequence-based reagent | mClock fw | This paper | qPCR primer | ATTGGTGGAAGAAGATGACAAGGA |
| Sequence-based reagent | mClock rv | This paper | qPCR primer | TACCAGGAAGCATAGACCCC |
| Sequence-based reagent | Bmal flox com fw | This paper | Genotyping PCR primer | ACTGGAAGTAACTTTATCAAACTG |
| Sequence-based reagent | Bmal flox com rv | This paper | Genotyping PCR primer | CTGACCAACTTGCTAACAATTA |
| Sequence-based reagent | Bmal flox mut fw | This paper | Genotyping PCR primer | CTCCTAACTTGGTTTTTGTCTGT |
| Sequence-based reagent | LysM cre mut rv | This paper | Genotyping PCR primer | CCCAGAAATGCCAGATTACG |
| Sequence-based reagent | LysM cre com fw | This paper | Genotyping PCR primer | CTTGGGCTGCCAGAATTTCTC |
| Sequence-based reagent | LysM cre wt rv | This paper | Genotyping PCR primer | TTACAGTCGGCCAGGCTGAC |
| Sequence-based reagent | Clock flox com fw | This paper | Genotyping PCR primer | CGCTGAGAGCCAAGACAAT |
| Sequence-based reagent | Clock flox com rv | This paper | Genotyping PCR primer | AGCTGGGGTCTATGCTTCCT |
| Sequence-based reagent | Per2 luc com fw |
This paper | Genotyping PCR primer | CTGTGTTTACTGCGAGAGT |
| Sequence-based reagent | Per2 luc wt rv | This paper | Genotyping PCR primer | GGGTCCATGTGATTAGAAAC |
| Sequence-based reagent | Per2 luc mut rv | This paper | Genotyping PCR primer | TAAAACCGGGAGGTAGATGAGA |
| Software, algorithm | CFX Manager | BioRad | V2.1 | |
| Software, algorithm | Chronolyse | In house generated software | V2.0 | |
| Software, algorithm | Chronostar | In house generated software | https://www.achim-kramer-lab.de/downloads.html | V3.0 |
| Software, algorithm | FlowJo | FlowJo | V10 | |
| Software, algorithm | GraphPad Prism | GraphPad | V9 | |
| Software, algorithm | photoNgraph | In house generated software | V2.0 | |
| Software, algorithm | ClockLab Analysis | Actimetrix |