Author response image 2. Effect of ARL4C mutants on pseudopod formation.

A,B,C, S2-CP8 cells were transfected with the indicated mutants of ARL4C-GFP and stained with the indicated antibodies (A).The percentages of cells with invasive pseudopods compared with the total number of cells were calculated (B). Cells were classified according to the number of pseudopods as indicated (C). A, The regions in the yellow dashed squares are shown enlarged in the left bottom images. The right bottom images are shown in a false color representation of fluorescence intensity. False color representations were color-coded on the spectrum. (B), Data are shown as the mean ± s.d. of 3 biological replicates. P values were calculated using one-way ANOVA followed by Bonferroni post hoc test. Scale bars in A, 10 μm. RFI, relative fluorescence intensity. n.s., not significant.