Tissue expression of PGAR. (A) RNA blot analysis of mouse PGAR (mPGAR) in adult mouse tissues. Ten micrograms of total RNA from tissues of 9-week-old male C57BL KS/J mice was fractionated on an agarose gel and transferred to nylon membranes. WAT, white adipose tissue; BAT, brown adipose tissue; EtBr, ethidium bromide staining. (B) Expression of human PGAR (hPGAR) RNA. Each lane contains 10 μg of total RNA prepared from surgical specimens (Brigham and Women's Hospital, Boston, Mass.).