FIG. 7.

Regulation of PGAR in mouse models of obesity and by nutritional changes and leptin administration. (A) Northern analysis of PGAR (10 μg RNA/lane) in white (WAT) and brown (BAT) fat tissues from 9-week-old obese (ob/ob) and diabetic (db/db) mice and their congenic controls. (B) PGAR expression in white adipose tissue of mice subjected to dietary restrictions. Eleven-week-old C57BL KS/J mice were divided into three experimental groups and were either allowed free access to food (lane 1) or subjected to 12 h of fast (lane 2) or 7.5 h of fast followed by refeeding (lane 3). The animals were sacrificed at the end of 12 h, and 10 μg of total RNA extracted from pooled white fat (n = 3 per group) was loaded on each lane. (C) PGAR expression in leptin-treated versus diet-restricted animals. Eight-week-old female C57BL/6J mice were divided into three groups (n = 5). The first two groups received intraperitoneal injections twice daily with either PBS or leptin. The third group, the pair-fed group, was diet-restricted to match the amount of food intake by the leptin-injected group and also received injections with PBS. The animals were sacrificed after 1 or 5 days, and 10 μg of total RNA from pooled white fat was analyzed by Northern blotting. See Materials and Methods for additional details.