Figure 4.

The effect of gp91^phox gene knockdown on XN-induced ROS. HL-60 cells were transfected with gp91^phox siRNA (si-gp91^phox) or its nontargeting control (si-NC) using methods and reagents described by the manufacturer (Santa Cruz, CA, USA) and incubated for an additional 24 hours, then treated with 10 μM XN for 1 h. Western blot (a) and QRT-PCR data (b) showed the gp91^phox protein and mRNA levels after transfected with siRNA, respectively (a, b). A representative flow cytometry histogram (c) and the related statistical results (d) were presented to show the effect of XN and DPI on HL-60 cells' ROS generation (n = 3; ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001 vs. control; #p < 0.05, “si-NC vs. si-gp91^phox”).