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. 2021 Feb 8;28(10-11):646–658. doi: 10.1038/s41434-021-00224-2

Fig. 2. Primary neuronal screen of sgNeuN#1–3 and two sgRNA scaffolds.

Fig. 2

a Vector map of the AAV9 transgene. Spacers #2 and #3 achieves robust reduction in b primary hippocampal neurons and c primary cortical neurons. Embryonic stage 16 primary neurons were transduced with 250 K AAV9 carrying sgRNAs constructs. Endogenous NeuN expression was normalized to GAPDH and then to Control, construct not expressing any sgRNA. Data represent means of n = 2 technical replicates. Refer to Fig. S4–S5 for representative gel images. FZ-Feng Zheng [Blue] and BH-Bo Huang [Red]. (color figure online).