Fig. 4. Injection of AAV-PHP.B-sgNeuN#2-BH significantly decreased NeuN protein level in the brain.

a Vector map of the AAV-PHP.B transgene. Injection of the high dose (2 × 10¹¹ GC) further decreased NeuN protein levels relative to the low dose (1 × 10¹¹ GC) injections in the b hippocampus, c cortex, and d spinal cord. AAV-PHP.B-sgNeuN#2-BH was bilaterally injected into P0 pups via ICV. CNS tissues were harvested at 6 weeks of age for protein quantification. All data points represent means ± SD of n samples noted above the x-axis and significance was tested with one-way ANNOVA followed by Tukey’s multiple comparison test; **P < 0.01, ***P < 0.001. Refer to Fig. S9–S11 for representative gel images.