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. 2021 Nov 4;9:753480. doi: 10.3389/fbioe.2021.753480

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Copyright © 2021 Mullins, Powers, Zobel, Clawson, Barnes, Draper, Zou, Binder, Dai, Zhang, Friese, Runnels, Jarrold and Thompson.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Anion Exchange High Performance Liquid Chromatography overlays of the fluorescence (FLD) and 260 nm absorbance (A₂₆₀) channels for both Human Ad5 Reference Material (Panels A & B) and mixtures of AdC68 low/high density viral particle fractions (Panels C & D). The Human Ad5 Reference Material data is a serial dilution and shows a similar response factor for both channels. The AdC68 mixtures give an increasing response factor on the 260 nm channel for samples of the similar particle # but differing particle density while that for the fluorescence channel is consistent among all samples.