TABLE 4.
Effects of transcription on recombination between direct-repeat substrates
| Strain | Gal80 |
lys2-Nhe
|
lys2Δ3′
|
Lys+ rate (10−5)a | Conversion (%) | Plasmid loss (%) | ||
|---|---|---|---|---|---|---|---|---|
| Promoter | Transcription | Promoter | Transcription | |||||
| SJR435 | + | LYS | Low | LYS | Low | 1.9 | 48 | 52 |
| SJR437 | − | LYS | Low | LYS | Low | 2.3 (1.2) | 48 | 52 |
| SJR432 | + | GAL | Low | GAL | Low | 2.0 | 41 | 59 |
| SJR434 | − | GAL | High | GAL | High | 71 (36) | 3 | 97 |
| SJR436 | + | GAL | Low | LYS | Low | 1.6 | 22 | 78 |
| SJR438 | − | GAL | High | LYS | Low | 23 (14) | 14 | 86 |
| SJR431 | + | LYS | Low | GAL | Low | 2.1 | 33 | 67 |
| SJR433 | − | LYS | Low | GAL | High | 12 (5.7) | 0 | 100 |
a
Fold increases over the rate in the corresponding Gal80+ strain are given in parentheses.