Fig. 3.

Number, size, and intensity of p62 puncta are increased in FTLD patient–derived fibroblasts. a Representative fluorescence microscopy images of staining with anti-p62 antibody (red) in fibroblasts of control, FTLD patient without (C9-), and FTLD patient with the C9-HRE (C9+). Nuclei were stained with DAPI (blue). b Quantification of number of p62 puncta. c Quantification of mean area of p62 puncta. d Quantification of intensity of p62 puncta. Data are shown as mean ± SEM and one-way ANOVA followed by Sidak’s multiple comparison test was performed (a, b) or median ± interquartile range and Kruskal–Wallis followed by Dunn’s multiple comparison test was performed (c, d). Only p values that were significant in the post hoc test are indicated in the graphs. Number of images analyzed n = 15 for control, n = 15 FTLD with C9-HRE, and n = 15 FTLD without C9-HRE. Data were obtained from one experiment and each datapoint represents a biological replicate. **p ≤ 0.01, ***p ≤ 0.001, ***p ≤ 0.001