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. 2021 Oct 21;99(11):skab300. doi: 10.1093/jas/skab300

Figure 6.

Figure 6.

Serum and liver TNF-α, IL-6, and haptoglobin responses in SCFP-treated calves compared with controls following viral–bacterial coinfection. Liver biopsies were collected 7 d prior to bovine respiratory syncytial virus infection and again at necropsy on day 10 after viral infection. RNA was isolated from liver samples and analyzed by qPCR for expression of (A) TNF-α, (B) IL-6, and (C) haptoglobin. Serum samples were collected on days 0, 6, 7, and 10 post-viral infection and analyzed using commercial ELISA kit for concentrations of (D) IL-6 and (E) haptoglobin. SCFP-treated (1 g/d SmartCare, 5 g/d NutriTek), n = 13; and controls, n = 13. One control calf was removed from the study on day 9 post-viral infection and is not included in the day 10 results. Data are presented as means ± SEM.