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. 2021 Aug 19;17(6):2193–2209. doi: 10.1007/s12015-021-10232-9

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Immunhistochemical analysis of SC-derived pancreatic organoids. d15 spheroids and d29 stem cell-derived organoids derived in 3D from the SC30 clone were fixed, sectioned and double-stained for (A) C-peptide (red) and insulin (green) or insulin (green) and glucagon, NKX6.1, SOX9 or PDX1 (B-E, all in red). A human non-diabetic pancreas was taken as control. Scale bars = 20 µm