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. 2021 Aug 26;17(6):2223–2234. doi: 10.1007/s12015-021-10220-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Induce UiPSCs-TEAD2, UiPSCs-TEAD4, and UiPSCs-ZIC3 differentiate to myocardial cells. a The cell morphology of induced myocardial cells from H1, UiPSCs, UiPSCs-TEAD2, UiPSCs-TEAD4 and UiPSCs-ZIC3 (bar: 200 μm). b FACS assay to detect the proportion of TNNT2+ cells after 15 days myocardial cells differentiation. c Immunofluorescence experiment to detect the expression of myocardial markers TNNT2 and α-actinin in differentiated myocardial cells. The nuclei were stained with DAPI (bar: 20 μm). d qPCR was used to detect the expression of pluripotency markers (NANOG) and myocardial markers (NKX2.5 and TNNT2) in differentiated cells and pluripotent cells. Data represented as mean ± SEM from three independent assays. *P < 0.05, **P < 0.01, ***P < 0.001, unpaired two tailed student-t-test