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. 2021 Nov 4;12:744745. doi: 10.3389/fphys.2021.744745

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Copyright © 2021 Qu, Lu, Bellve, Lifshitz and ZhuGe.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Changes in gene expression in adenomyotic uteri in neonatal and adult mice. mRNA for each gene was quantified by qPCR; the expression level was calculated using the 2^{– Δ} ^Ct method and was normalized against β–actin. Genes are grouped and plotted in panels (A–C), according to their relative expression levels. Note that α-SMA, Col1a1, Col3a1, Esr1, and Oxtr1 were changed in the same direction in both age groups, while E-cadherin and Tgbr1 were up-regulated only at PND14, and β-catenin was changed in the opposite direction between two groups. N.S., no statistical significance, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 with unpaired Student’s t-test (N = 3 repeats in A–C with 3 (adult) or 9 (neonate) mice in each repeat). Ctrl, vehicle treatment; Adeno, tamoxifen treatment; PND, postnatal day.