TABLE 1.
Effect of repeat length on hnRNP D bindinga
DNA sequence | Length (nt) | KD (nM) |
---|---|---|
GACGTC(TTAGGG)1CGAT | 16 | >400 |
GACGTC(TTAGGG)2CGAT | 22 | 150 |
GACGTC(TTAGGG)3CGAT | 28 | 60 |
GACGTC(TTAGGG)4CGAT | 34 | 29 |
GACGTC(TTAGGG)5CGAT | 40 | 25 |
GACGTC(TTAGGG)6CGAT | 46 | 21 |
hnRNP D was incubated with the indicated radiolabeled oligonucleotides, and the complexes were resolved by gel electrophoresis.