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. 2021 Nov 17;12:6645. doi: 10.1038/s41467-021-26966-6

Table 1.

Summary of scRNA-seq samples.

Time point No. of embryos Somites Cell viability Cell number (captured) Mean reads/cell Median genes/cell Tissue
Mesp1Cre; ROSA26-GFPf/+
 E8.0 7 3–4 90% 7581 58,422 4380 Half of the body
 E8.25 5 6–7 95% 2956 151,357 5363 Half of the body
 E9.5 6 19–22 80% 4055 80,919 4036 PA and heart
 E9.5 2 21–22 81% 7418 60,500 4106 PA and heart
 E10.5 4 30–31 80% 8070 52,326 3673 PA and heart
Mesp1Cre;ROSA26-GFPf/+;Tbx1f/f
 E9.5 1 22 83% 5157 65,997 5015 PA and heart
 E9.5 2 24 92% 10,281 43,868 3617 PA and heart
Tbx1Cre/+; ROSA26-GFPf/+
 E8.5 7 8–9 88% 1434 136,347 3683 Half of the body
 E9.5 10 19–21 87% 5453 54,317 3994 PA and heart with back
Tbx1Cre/f; ROSA26-GFPf/+
 E8.5 4 9–10 92% 1090 140,352 4374 Half of the body
 E9.5 3 19–22 97% 6267 54,892 3561 PA and heart with back
Tbx1−/− vs wild-type
 E9.5 WT 4 22–23 89% 12,050 37,218 3757 PA and heart with back
 E9.5 KO 4 20–23 92% 11,504 37,646 4138 PA and heart with back