Table 4.
Dose response for selected natural product compounds. Doubling time (DT) (minutes/cell division), standard error of the mean (SEM), percent change, and number of biological replicate cultures tested (n) for wild type (WT), tor1Δ, and fpr1Δ cells
| Treatment | WT | tor1Δ | fpr1Δ | ||||||
|---|---|---|---|---|---|---|---|---|---|
| DT (SEM) | % change | n | DT (SEM) | % change | n | DT (SEM) | % change | n | |
| 1% DMSO | 80.0 (2.8) | - | 3 | 81.1 (1.8) | - | 3 | 85.9 (1.3) | - | 3 |
| Berberine 1 µg/mL | 81.2 (1.2) | 1.5 | 3 | 85.2 (0.4) | 5.1 | 3 | 89.5 (0.3) | 4.2 | 3 |
| Berberine 10 µg/mL | 93.7 (1.4) | 17.0 | 3 | 88.3 (3.2) | 9.0 | 3 | 98.1 (4.8) | 14.2 | 3 |
| Berberine 50 µg/mL | 146.3 (4.2) | 82.8* | 3 | 135.7 (1.0) | 67.4* | 3 | 143.1 (0.9) | 66.7* | 3 |
| 1% DMSO | 83.1 (1.6) | - | 3 | 82.3 (0.7) | - | 3 | 92.0 (1.4) | - | 3 |
| Celastrol 5 µM | 86.0 (0.8) | 3.4 | 3 | 85.8 (1.1) | 4.3 | 3 | 92.4 (0.7) | 0.4 | 3 |
| Celastrol 10 µM | 92.7 (2.1) | 11.5 | 3 | 92.4 (1.2) | 12.3* | 3 | 102.0 (0.8) | 10.9* | 3 |
| Celastrol 100 µM | 94.2 (2.8) | 13.4 | 3 | 92.5 (4.2) | 12.4 | 3 | 100.8 (2.4) | 9.5 | 3 |
| 1% DMSO | 81.6 (0.8) | - | 3 | 79.1 (1.4) | - | 3 | 90.7 (1.6) | - | 3 |
| Isoliquiritigenin 1 µg/mL | 79.3 (1.2) | -2.8 | 3 | 77.7 (1.2) | -1.8 | 3 | 87.1 (1.1) | -4.0 | 3 |
| Isoliquiritigenin 10 µg/mL | 82.4 (2.1) | 1.0 | 3 | 81.3 (1.1) | 2.8 | 3 | 92.4 (2.5) | 2.0 | 3 |
| Isoliquiritigenin 50 µg/mL | 98.7 (2.9) | 21.0 | 3 | 99.0 (1.7) | 25.2* | 3 | 113.6 (2.5) | 25.3* | 3 |
*p < 0.05, compared to 1% DMSO, Welch’s (unequal variance) t-test