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. 2021 Nov 4;12:753548. doi: 10.3389/fphar.2021.753548

FIGURE 2.

FIGURE 2

EGCG attenuates H2O2-induced ECM degradation in NPCs. (A) NPCs were pretreated with EGCG for 4 h and then stimulated with H2O2 for 24 h, qPCR results showing expression levels of Collagen II, Aggrecan, MMP13, and ADAMTS5. (B) Western blot findings of protein expression levels. (C) Quantifications of signal intensities. GAPDH was the loading control. (D) Immunofluorescence of Collagen II as well as MMP13 in NPCs. Data are shown as mean ± SD (n = 3). * p < 0.05, ** p < 0.01.