FIGURE 6.

EGCG attenuates H₂O₂-induced apoptosis in a Bmal1-dependent manner in rat NPCs. Rat NPCs were transfected with si-Bmal1 or si-ctrl for 48 h, followed by pretreatment with or without 20 μM EGCG for 4 h and with H₂O₂ for an additional 24 h. (A) Apoptosis of NPCs in each treatment group was examined by TUNEL assay, and (B) the TUNEL-positive cells were quantified. (C) Cleaved caspase3, Bax as well as Bcl-2 levels were measured by western blot analysis. (D) Quantification of the blots. Data are shown as the mean ± SD (n = 3). ^* p < 0.05, ^** p < 0.01.