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. 2021 Nov 17;11:22377. doi: 10.1038/s41598-021-01883-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Mucosal IgA antibody response related to different immunization protocols (D0) against M. hyopneumoniae along the experimental period. Dots represent the mean values of each group in each day of sampling. Positive S/P values > 0.4. (b) Serum IgG antibody response obtained in different immunization protocols (D0) against M. hyopneumoniae along experimental period. Piglets challenged with M. hyopneumoniae on D49 (red arrows). Dots represent the mean values of each group in each day of sampling. Positive S/P values > 0.3. Kruskall-Wallis test was used.