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. 2021 Nov 17;11:22377. doi: 10.1038/s41598-021-01883-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Bar graphs representing the fold change of cytokine gene expression (Fold Change mean ± sd) in lung lesion samples of five pigs per group, 28 days after experimental infection with M. hyopneumoniae strain 232, previously submitted to different immunization protocols. Target gene expression was normalized based on rpl-4 gene expression. No statistical differences were found between groups (Kruskall-Wallis test).