. 2021 Nov 17;11:22377. doi: 10.1038/s41598-021-01883-2

Table 3.

Details of the primer sequences of cytokines used for quantitative SYBR Green real-time PCR amplification. The rpl-4 gene was used as reference.

Target gene	Primer sequence	Access number	qPCR efficiency (%)	Slope	R²	Amplicon size (bp)	Amplicon melting temperature (°C)
rpl-4	(F)CAAGAGTAACTACAACCTTC	NC_010443.5	96.79	3.401	0.994	122	75
rpl-4	(R)GAACTCTACGATGAATCTTC	NC_010443.5	96.79	3.401	0.994	122	75
IL-8	(F)AGGAAAAGTGGGTGCAGAAG	NM_213867.1	97.67	3.379	0.991	190	78
IL-8	(R)CAACCCTATGTCTGACCAGC	NM_213867.1	97.67	3.379	0.991	190	78
IFN-γ	(F)ATTGGAAAGAGGAGAGTGAC	NM_213948.1	96.54	3.408	0.997	168	76.5
IFN-γ	(R)CATTCAGTTTCCCAGAGCTA	NM_213948.1	96.54	3.408	0.997	168	76.5
IL-4	(F)AGAGCTCTATTCATGGGTCT	NM_214123.1	99.88	3.325	0.997	209	82
IL-4	(R)CTTCTCCGTCGTGTCTCT	NM_214123.1	99.88	3.325	0.997	209	82
TGF-β	(F)GGATACCAACTACTGCTTCA	NM_214015.2	100.85	3.302	0.996	228	83
TGF-β	(R)TTGTACAGAGCCAGGACTT	NM_214015.2	100.85	3.302	0.996	228	83