FIG. 6.

BNIP3 induces rapid plasma membrane permeability but not PS externalization. (A) Untransfected and BNIP3-T7-transfected 293T cells were harvested at 2, 4, 8, and 12 h posttransfection and stained with PI. PI⁺ cells are expressed as the mean ± SE of three or four experiments for each time point. (B) Untransfected 293T cells (C) and 293T cells transfected with BNIP3-T7 (BNIP3), BNIP3ΔTM-T7 (ΔTM), tBID-FLAG (tBID), BAX, or caspase 9/Apaf-1 (C9/A) were harvested at 12 h posttransfection and stained for annexin V and PI. Cells that were gated as PS⁺ PI⁻ are expressed as the mean ± SE of three independent experiments.