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. 2021 Nov 2;49(20):11765–11777. doi: 10.1093/nar/gkab932

Figure 4.

Figure 4.

Functional investigation of the newly annotated FAR1 gene, Oryza sativa FAR1 related to Fertility (OFF). (A) qRT-PCR analysis of OFF using seven rice tissues is illustrated. Relative expressions were calculated using the rice ubiquitin 5 gene (OsUbi5, LOC_Os01g22490). Three biological replicates were used for the analysis, and error bars indicate standard deviation. (B) Subcellular localization of the OFF-encoded protein in tobacco epidermal cells is shown. The OFF-GFP protein localized to the nucleus, merging exactly with the nuclear marker protein, OsRH36-mCherry (scale bar = 20 μm). (C) Gene structure of OFF and the sequence of its knockout mutant, off-1. The CRISPR/Cas9-targeted region is shown by dotted lines. Black arrows indicate primer regions for qRT-PCR and genotyping of the off-1 mutant. WT represents wild-type plant (O. sativa ssp. japonica cv. Dongjin). (Dand E) Phenotypes of the WT and off-1 mutant plants during the ripening stage. (F) Fertility of the WT and off-1 mutant plants. Five panicles from each of three replicates were analyzed. Error bars indicate standard deviation. *** means P < 0.001.