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. 2021 Nov 1;49(20):11550–11559. doi: 10.1093/nar/gkab946

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© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — HU did not reduce the variation of looping probabilities among different tethers. (A) The calculated looping probabilities of individual tethers, exposed to 2.5 nM and a range of HU protein concentrations, are summarized in a box–whisker plot. The whiskers span the entire range of probabilities of the ensemble of DNA tethers monitored in each condition. As the HU concentration was titrated from 0 to 1056 nM, the average looping probability progressively increased from 25 to just above 80. Note, however, that at each concentration of HU, the looping probabilities for individual DNA tethers varied from 0 to 100. The upper and lower borders of the boxes indicate the upper and lower quartiles, respectively. The midline and cross of each box indicate the median and average of the distribution of looping probabilities, respectively. (B) Representative temporal records of the TPM excursion parameter with 2.5 nM LacI and 66 nM HU are ranked by looping probability from 0 (top) to 1 (bottom). Unlooped states have larger excursions (tall/red signal) and looped states have narrower excursions (short/blue and green signal). The actual values are encoded using the color scale at right.

Inline graphic — HU did not reduce the variation of looping probabilities among different tethers. (A) The calculated looping probabilities of individual tethers, exposed to 2.5 nM and a range of HU protein concentrations, are summarized in a box–whisker plot. The whiskers span the entire range of probabilities of the ensemble of DNA tethers monitored in each condition. As the HU concentration was titrated from 0 to 1056 nM, the average looping probability progressively increased from 25 to just above 80. Note, however, that at each concentration of HU, the looping probabilities for individual DNA tethers varied from 0 to 100. The upper and lower borders of the boxes indicate the upper and lower quartiles, respectively. The midline and cross of each box indicate the median and average of the distribution of looping probabilities, respectively. (B) Representative temporal records of the TPM excursion parameter with 2.5 nM LacI and 66 nM HU are ranked by looping probability from 0 (top) to 1 (bottom). Unlooped states have larger excursions (tall/red signal) and looped states have narrower excursions (short/blue and green signal). The actual values are encoded using the color scale at right.