Figure 2.

Tetramethylenedisulfotetramine (tetramine or TETS) triggers rapid overall changes in neuronal network synchronous Ca²⁺ oscillation (SCO) patterns by stabilizing high amplitude SCO events (HASE) and eliminating low amplitude SCO events (LASE). Graphic representation of the experimental protocol (inset). A, representative SCO traces before (Baseline) and after addition of TETS (3.0 µM) illustrate a rapid but transient rise in cytoplasmic Ca²⁺ (phase I response) and subsequent alterations in 2 distinct populations of SCO events: HASE and LASE (phase II response). Vehicle (0.01% DMSO) showed no significant influences on SCO during either Phase of recording. B, Magnified traces recorded during Baseline, phases I and II; Orange box: Baseline activity showing the green-dashed line is the cut-cutoff thresholds for binning LASE (ΔF/F0 ≤ 0.3) and the blue-dashed line is the binning cutoff for HASE. Purple box: typical phase II response after applying 3 μM TETS, which eliminates all LASE.