Figure 3.

Tetramethylenedisulfotetramine (tetramine or TETS; 3 µM) decreases overall total SCO frequency and increases SCO amplitude by stabilizing high amplitude SCO events (HASE) and suppressing low amplitude SCO events (LASE). Upper panel shows the experimental protocol for recording baseline and phases I and II recordings postaddition of TETS (3.0 µM). A, Total SCO frequency (red bars), HASE (blue bars) and LASE (green bars) and (B) total amplitude are measured after addition of TETS and normalized to Vehicle control (0.01% DMSO). Total frequency and amplitude are measures of all events (HASE+LASE). All responses to TETS are normalized to respective vehicle controls and reported as mean ± SEM percent change (n = 10–15 wells measured on 3 independent culture days). ANOVA with Tukey post hoc correction *p < .05; **p < .01; ***p < .001.