Figure 9.

Comparative analysis of 4 neuroactive steroid (NAS) for normalizing tetramethylenedisulfotetramine (TETS)-triggered electrical spike activity (ESA) patterns in the presence of midazolam (MDZ). Mean firing rate (A), mean burst frequency (B) and area under cross correlation (C) of neuronal networks after introducing TETS (1 µM) in the presence of combinations of MDZ (0.1 µM) plus 0.1 µM allopregnanolone, ganaxolone, XJ-42, or eltanolone. ESA events are acquired for 10 min before and after addition of compounds and normalized to their respective baselines. Means of each ESA parameter for each dual intervention were compared with the respective timeframe recorded from Veh control. One-way ANOVA with additional correction (Tukey) for post hoc multiple comparisons are applied to determine the statistical differences. Data represent mean ± SEM of data from 5 to 18 wells. *p < .05; **p < .01; ***p < .001 compared with Veh (no exposure to TETS or MDZ/NAS).