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. 2021 Oct 20;49(20):11537–11549. doi: 10.1093/nar/gkab935

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© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 4. — Bimodal distribution of reporter fluorescence in the presence of decoy sites. (A) Flow cytometry analyses of RU2079 (P_phoA-yfp) carrying different decoy plasmids. Every decoy plasmid contains a constitutively expressed cfp gene. The vertical axis shows CFP fluorescence that reflects plasmid copies while the horizontal axis shows the reporter YFP fluorescence. Dashed rectangles highlight the cell populations with activated YFP fluorescence, and correlations between CFP and YFP fluorescence for these activated populations are indicated by the Spearman correlation coefficient r. (B and C) Histograms of P_phoA-yfp fluorescence for the autoregulated WT (RU2079) and non-autoregulated LAC (RU1988) strain.