Figure 6.

Washout experiments of T. cruzi-infected cardiac cell cultures. Number of culture-released trypomastigotes as a function of incubation time. Infected cultures (Y strain) were incubated for 168 h with Cpd1 or benznidazole (Bz) and then another 168 h with drug-free medium before assay readout. Data represent mean ± SD of two independent experiments. The grey dotted line indicates the timepoint at which compound exposure is halted by changing to drug-free medium. Statistically significant values between control and treated groups were calculated by Welsh multiple non-parametric t-test (P < 0.05, 95% CI) and P values ranging 0.000009 ≤ P < 0.002.