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. 2021 Nov 17;4:1296. doi: 10.1038/s42003-021-02773-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Mice were implanted with 200,000 B16F10 cells (hereby referred to as B16 or B16 melanoma) injected intradermally or 200,000 4T1 cells injected subcutaneously (n = 5 mice/group). Tumors were harvested 10 days post tumor challenge and fixed for immunohistochemistry (IHC) prior to staining with picrosirius red (PR) and alpha-smooth muscle actin (αSMA). a Representative cross sections of B16 melanoma (left) and 4T1 breast (right) stained with hematoxylin and eosin (H&E, top), PR (middle), and αSMA (bottom). b Bar graphs demonstrate quantification of the staining of either PR or αSMA ± standard deviation (SD) following analysis by Halo software with supervision from a pathologist. *p < 0.05; ****p < 0.001.