Fig. 3. ONECUT1 binds to regulatory gene clusters relevant for pancreas and endocrine development.

a Distribution of ONECUT1 ChIP-seq peaks at GT and PP stages relative to genomic regions. b Schematic of the binding enrichment analysis evaluating whether ONECUT1-bound genes (binding observed 20 kb upstream or downstream of the respective promoter regions) are enriched in particular RNA-seq clusters. c Binding enrichment (z-score) test of ONECUT1 (ChIP-seq, PP stage) in genes of the RNA-seq clusters of HUES8 cells (z-test; one-sided). d, e Enrichment analysis (GREAT³⁶) of ONECUT1-bound genes (ChIP-seq, PP stage) (Fischer Exact test; one-sided). f Binding enrichment (z-score) test of ONECUT1 (ChIP-seq, PP stage) in genes of the RNA-seq clusters of CyT49 cells (z-test; one-sided). Significance is indicated above bars (Fischer Exact test; one-sided). g Binding enrichment test of ONECUT1 (ChIP-seq, PP) for MEL1 RNA-seq specific gene clusters. Data show binding score (z-score) with p-value (z-test; one-sided). h Overlap of ONECUT1 ChIP-seq peaks (PP stage, own data set) with peaks from other TFs (previously published at GSE54471, GSE149148, or NKX6.1; own data set). For the other TFs, only the number of binding peaks overlapping with ONECUT1 is shown.