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. 2021 Nov 16;12(12):1086. doi: 10.1038/s41419-021-04369-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A The expression level of TMEM116 in mouse embryonic lungs were examined by RT-PCR and western blot analyses. B Immuno-fluorescence (IF) staining analysis showed TMEM116 expression in E14.5, PN1 and adult mouse lungs. C Double IF staining analysis of TMEM116 and p63, β-tubulin, CC10, PGP9.5, T1-α, and Spc respectively. D IF staining analysis of TMEM116 and GFP in Dermo1-cre; ROSA^mTmG mice. Representative images from three independent experiments are shown above. Scale bar: 50 μm.