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. 2021 Oct 13;49(20):11855–11867. doi: 10.1093/nar/gkab879

Figure 3.

Figure 3.

Requirement of tRNA m1A58 for plus-strand strong-stop by M-MLV RT and HIV-1 RT using retroviral primer tRNAs in vitro. (A) Investigation of the requirement for m1A58 in tRNAPro for reverse transcription-stop during plus-strand cDNA synthesis by M-MLV RT on primer tRNAProin vitro. In addition to dATP and dTTP, ddGTP was used to terminate reverse transcription at tRNAPro C56. Mouse embryonic fibroblast (MEF) RNA was used to confirm that reverse-transcription stops at m1A58 of wild-type mouse tRNAPro. (B) Investigation of the necessity of m1A58 in the HIV-1 primer tRNA3Lys for reverse transcription-stop during plus-strand cDNA synthesis by HIV-1 RT in vitro. In addition to dATP, dTTP and dCTP, ddGTP was used to terminate reverse transcription at tRNA3Lys C56.