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tRNA modification activity of PUS7 variants. (A) Substitutions G128R in the catalytic domain and D503Y in the C-terminal insertion domain are indicated in dotted spheres and residues 398–459, the helix-turn-helix motif, which was deleted, are highlighted in (magenta). (B) Single turnover pseudouridylation reactions were performed by mixing 100 nM of [3H-C5] uridine-labeled tRNAGln with 1000 nM of the different PUS7 variants. The error bars represent one standard deviation (n = 3−6).
