Figure 7.

Comparison of PUS7 activity modifying tRNA^Gln or tRNA^Ala. (A) 18-nucleotide 5′ fragment of cytoplasmic (cy) tRNA^Ala and micro-variants of cytoplasmic tRNA^Ala that only contain the acceptor and T-arm including a loop with the PUS7 consensus sequence; the putative target uridine is shown in yellow. The secondary structures have been predicted using mFold. (B) Multiple-turnover tritium release assays of PUS7(Δ1–98) with cytoplasmic tRNA^Gln, tRNA^Ala, its micro-RNA variant and a short 18-nucleotide 5′ fragment of tRNA^Ala. The error bars represent one standard deviation (n = 4).