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. 2021 Oct 28;49(20):11643–11652. doi: 10.1093/nar/gkab899

Figure 4.

Figure 4.

Loss of FAN1 and EXO1 has an additive effect on expansion. (A) Typical repeat PCR profiles from small intestine of 6-month old WT, Fan1-/-, Exo1-/- and Fan1-/-Exo1-/- double mutant mice matched for initial repeat number. The dotted line represents the size of the original inherited allele as ascertained from the tail DNA taken at weaning (3-week tail). The numbers associated with the samples from the small intestine indicates the number of repeats added to alleles in that organ during the lifetime of the mouse. (B) Comparison of the number of repeats added in different organs of age and size matched WT, Fan1-/-, Exo1-/- and double mutant Fan1-/-Exo1-/- mice. The data represent the average of nine WT, five Exo1-/-, five Fan1-/- and six double mutant Fan1-/-Exo1-/-male mice with 161–168 repeats. The numbers above the bars in the small intestine indicate the average number of repeats added relative to wildtype for all three of the mutant genotypes. The error bars indicate the standard deviations of the mean. Significance was assessed using a RM two-way ANOVA (genotype and tissue as variables) with correction for multiple testing as described in the Materials and Methods. The adjusted P values of genotype effect are listed in the table below.