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. 2021 Oct 28;49(20):11653–11665. doi: 10.1093/nar/gkab942

Figure 6.

Figure 6.

CST inhibits telomerase extension independent of bound pol α-primase. (A) Western blot analysis of WT CST purified in standard 150 mM NaCl or in 300 mM NaCl. Top blots probed with antibodies to CTC1 and to the large subunit of pol α. Bottom blots probed with antibodies to STN1, the small subunit of pol α, and the two subunits of primase (Prim 1 and 2). Wedges indicate successive two-fold dilutions of protein. (B) Pulse-chase experiment with WT CST purified in 300 mM NaCl. (C) Pulse-chase experiments with WT CST purified in 150 mM NaCl (left lanes) and with g2.1 mutant CST purified in 150 mM NaCl (right lanes). (D) Radioactivity incorporated into telomerase products as a function of reaction time without chase (No CST) or in the pulse-chase experiments of panel (B) and left half of (C) (300 and 150, respectively). (E) Long extension products (≥10 repeats) as a fraction of total incorporation in the same pulse-chase experiments. Data points are connected to aid visualization.