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. 2021 Jul 16;30(23):2347–2361. doi: 10.1093/hmg/ddab199

Figure 4 .

Figure 4

Exposure to miR-339-5p-depleted exosomes alters mitochondrial stress response, but not target gene levels. Following 48 h knockdown with anti-miR-339-5p, miR-339-5p is depleted in (A) DMD-iCMs and (B) DMD-exos. N = 3 independent experiments; *P < 0.05 versus Vehicle. Following 48 h exposure to miR-339-5p-depleted exosomes, levels of target genes MDM2 and GSK3A (C) remain unchanged at baseline and (D) remain unchanged following stress. (E) Stress-induced ROS levels also remain the same following 48 h exposure to miR-339-5p-depleted exosomes. (F) Exposure to miR-339-5p-depleted exosomes results in protection against stress-induced mitochondrial injury and (G) increases in cell death, indicating miR-339-5p may be involved in modulating mitochondrial responses to stress. N = 3 independent experiments; *P < 0.05 versus Vehicle.