FIGURE 5.

VSD-I in the activated-state structure and resting-state model. (A,B) Intra-membrane views at VSD-I in the activated (A) and resting (B) states. Side chains of some w54 residues and their contacts are indicated with green and red labels, respectively. Dashed lines indicate displacement of K²²⁸ upon activation of VSD-I. (C) Matrix of contacts variants. To indicate contacts, which may switch upon activation of VSD-I, each cell is labeled “C”, “M” or “B” depending on whether the contact is found only in the cryo-EM structure, only in the resting-state model, or both in the cryo-EM structure and the model. Overwhelming majority of PDVs and their contact variants are associated with BrS1. See section VSD-I for more details.