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Workflow chart. General strategy in three steps. First step, the exosomes isolation and characterization showed CD63 and CD81 tetraspanin expression in serum exosomes from DR-TB patients. Second step, extraction of both host-miRNAs and M. tuberculosis-RNA. Third step, miRNAs differentially expressed in serum exosomes from MDR-TB patients at T(0) and T(12). In red, the 8 miRNAs selected for individual analysis. BioRender, publication and licensing rights (QX22QHOTQ4).
