Fig. 2.

EGCG pretreatment enhanced adaptive autophagy in Dox-injured cardiomyocytes. A Dox-induced autophagic flux evaluated by the difference in the P62 and LC3 levels between NRCMs subjected or not to treatment with 100 nM BafA1. B Dox-induced autophagic flux evaluated by the difference in the P62 and LC3 levels between H9C2 cells subjected or not to treatment with 100 nM BafA1. C Western blot bands showing LC3 and P62 protein expression and the relative signal intensities in NRCMs pretreated with 20 μM EGCG for 24 h and then exposed to 1 μM Dox, and in cells subjected or not to 20 μM EGCG and 5 mM 3-MA co-treatment for 48 h; the below findings were also obtained using this method. D Western blot bands showing LC3 and P62 protein expression and the relative signal intensities in H9C2 cells. e NRCM images stained with MDC. f NRCM images stained with LysoTracker Red DND-99. Values are mean ± SD from three individual experiments. NS, nonsignificant. *p < 0.05 compared with the indicated groups. **p < 0.01 compared with the indicated groups. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)