Skip to main content
. 2021 Nov 11;48:102185. doi: 10.1016/j.redox.2021.102185

Fig. 3.

Fig. 3

EGCG pretreatment upregulated AMPKα2 and promoted TCA cycle activation in Dox-injured cardiomyocytes. A Western blot bands showing total and phosphorylated AMPKα2, mTOR, and ULK1 expression and the relative intensities of p-AMPKα2/AMPKα2, p-mTOR/mTOR, and p-ULK1/ULK1 expression in NRCMs pretreated with 20 μM EGCG for 24 h and then exposed to 1 μM Dox, and in cells subjected or not to 20 μM EGCG and 5 μM compound C co-treatment for 48 h; the below findings were also obtained using this method. B The relative intensity of p-ACC/ACC and the contents of α-KG and Suc in NRCMs. C Western blot bands showing total and phosphorylated AMPKα2, mTOR, and ULK1 expression and the relative intensities of p-AMPKα2/AMPKα2, p-mTOR/mTOR, and p-ULK1/ULK1 expression in H9C2 cells pretreated with 20 μM EGCG for 24 h and then exposed to 1 μM, and in cells subjected or not to 20 μM EGCG and pAD/AMPK-shRNA co-treatment for 48 h; the below findings were also obtained using this method. D The relative intensity of p-ACC/ACC and the contents of α-KG and Suc in H9C2 cells. Values are mean ± SD from three individual experiments. NS, nonsignificant. **p < 0.01 compared with the indicated groups.