Fig. 4.

EGCG pretreatment improved redox equilibrium and lipid metabolism in Dox-injured cardiomyocytes. A Images of DHE/MitoSOX probe-stained H9C2 cells and the relative fluorescence intensity of DHE/MitoSOX in H9C2 cells pretreated with 20 μM EGCG for 24 h and then exposed to 1 μM Dox, and in cells subjected or not to 20 μM EGCG, 2 μM Fer-1, and 10 μM MitoTEMPO co-treatment for 48 h; the below findings were also obtained using this method. B The MDA and 4-HNE contents in H9C2 cells. C The levels of GSH, GSSG, and the GSH/GSSG ratio in H9C2 cells. Values are mean ± SD from three individual experiments. **p < 0.01 compared with the indicated groups.